DNA Testing in Felines

Earlier this year, I had the situation arise where a female of mine was pregnant and I knew that she had been exposed to 2 different males. While one was young and I had never seen him breed, the fact was still there that he was running loose in the home with this girl. I began a search to find out about DNA testing and what was involved in the process. Since then, I have had many requests for the information that I obtained and questions about the process. Below is my experience and this article is intended to help with others that may need the information.

I decided to use the University of California at Davis. I first called them on the phone and ordered my “swab” kits. In my case, I had 1 mother, 2 “possible” fathers, and 5 kittens. Within a few days, a box arrived with numerous brushes and some paperwork. They will send you 2 swabs for each cat/kitten to be tested. There was no charge for these swabs. Each swab comes in a sterile paper/plastic wrapper that has a label on it. You will need to write each kittens name (or assign them a number if you do not have names) on each wrapper remembering that there is 2 wrappers for each cat/kitten. I used 16 swabs (2 for mother, 4 for the fathers, and 10 for the kittens). When you open the wrapper, make sure you only open a small part at the top because you will be reinserting the swab into the original wrapper and mailing it back.

When all your wrappers are labeled, it is time to swab the cats. Take each cat/kitten and hold them in your lap. Place the brush into the mouth and gentle brush the outer gums and/or cheek. You do not need to press hard and you will not “see” anything on the brush when you are finished. Do this for approximately 10 seconds for each cat/kitten. When you are finished, place the brush back into the original wrapper and repeat with the next brush on the same cat/kitten. When you are finished, move on to the next one. One important note is to make sure the cat/kitten has not eaten anything recently.

Once all cats/kittens have been swabbed, place all your swabs in their wrappers into a box and seal the box. Take your form and fill it out to match your swabs. Place the form into the box with the swabs, along with your check for services, and mail back to UC Davis. I have attached a sample form to be used as a guide. In approximately 3 weeks, you will receive a document back for each cat tested. Each kitten will have his or her own paper and at the bottom it will state who the “match” was. Once a cat/kitten is tested, a number is then assigned to that cat and should you ever need to use it again, you will not need to reswab the cat. Just simply place the number on the line next to the cats name and they will be able to research it from their database and use it.

The cost to include is stated at the bottom of the application, but it is $40.00 per cat tested. They do offer a litter fee of $300.00 plus $40.00 for additional sires. If you are only testing a few, and the cost is cheaper to not use the litter fee, then use the lesser amount. Litter fee is more reasonable when you have a litter of 7 or more kittens.

The contact information is:

http://faculty.vetmed.ucdavis.edu/faculty/lalyons/Sites/catfingerprint.htm

University of California – Davis
Veterinary Genetics Laboratory-Cat DNA testing
Old Davis Road/One Shields Ave.
Davis, CA 95616-8744
(530)752-2211

actual form currently in use by UC Davis (click to enlarge image):

This is a very strict and confidential service. If you provide a fax number on your application, they will FAX you a copy of the results and then mail you a copy also. If you do not provide a number at the time of submitting an application, they WILL NOT allow you do so later and NO information will be given out on the telephone.